Three weeks into the new term and we have been going through the basic skills required to make accurate dilutions using automatic pipettes. Using a range of microbiological dyes, UTC students from Y10-13 are coming to terms with the "reliability" of these pipettes for delivering microlitre volumes in order to produce high quality standard curves using the Beer Lambert Law. The main aim of these experiments is to develop ownership of your experimental results and to keep practising until you get a result that is "trustworthy" and one that is unequivocal. At this stage on your journey, the most important thing is to ensure you understand the significance of each step in a protocol and that you appreciate when to use (in this case) a P1000, a P20 etc in preparing dilutions or reaction mixtures for PCR etc.
Key Words: Pipette, Gilson, standard curve, dilution, volumetric.